Journal
NATURE METHODS
Volume 9, Issue 9, Pages 907-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2131
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Funding
- Canadian Institutes for Health Research [MOP-77688]
- Canada Research Chairs program
- Danish Agency for Science Technology and Innovation
- Canada Foundation for Innovation
- British Columbia Knowledge Development Fund
- BC Proteomics Network
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Interactomes are often measured using affinity purification-mass spectrometry (AP-MSMS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MSMS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.
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