Journal
NATURE METHODS
Volume 10, Issue 1, Pages 54-U129Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2250
Keywords
-
Categories
Funding
- US National Center for Research Resources [5P41RR011823-17]
- National Institute of General Medical Sciences [8P41GM103533-17]
- National Institute of Diabetes and Digestive and Kidney Diseases [R01DK074798]
- National Heart, Lung, and Blood Institute [RFP-NHLBI-HV-10-5]
- National Institute of Mental Health [R01MH067880]
Ask authors/readers for more resources
Two major challenges in proteomics are the large number of proteins and their broad dynamic range in the cell. We exploited the abundance-dependent Michaelis-Menten kinetics of trypsin digestion to selectively digest and deplete abundant proteins with a method we call DigDeAPr. We validated the depletion mechanism with known yeast protein abundances, and we observed greater than threefold improvement in low-abundance human-protein identification and quantitation metrics. This methodology should be broadly applicable to many organisms, proteases and proteomic pipelines.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available