Journal
NATURE METHODS
Volume 9, Issue 9, Pages 913-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2137
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Funding
- Helen Hay Whitney Foundation
- US National Institutes of Health-National Cancer Institute [1R21CA160080]
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We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis.
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