Journal
NATURE METHODS
Volume 9, Issue 5, Pages 467-U76Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1930
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Funding
- Australian National Health and Medical Research Council [566640]
- Miegunyah Trust
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We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
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