Journal
NATURE METHODS
Volume 8, Issue 4, Pages 315-U54Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1580
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Funding
- Else-Kroner Fresenius Stiftung fellowship
- California Institute for Regenerative Medicine [RT1-01108, TR1-01250, CL1-00502]
- US National Institutes of Health [R21 MH087925]
- Bill and Melinda Gates Foundation
- Esther O'Keeffe Foundation
- Millipore Foundation
- US National Institutes of Health National Institute of Child Health and Human Development
- New York State Stem Cell Science grant [N08T-060]
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Pluripotent stem cells (PSCSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCSCs is cell transmission through the germline, but for human PSCSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.
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