Journal
NATURE METHODS
Volume 9, Issue 2, Pages 159-172Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.1808
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Funding
- Bio-X
- US National Institute of Mental Health [1F32MH088010-01]
- International Fulbright Science and Technology Award
- Stanford Graduate Fellowship
- National Institute of Mental Health
- National Institute on Drug Abuse
- National Institute of Neurological Disorders and Stroke
- Howard Hughes Medical Institute
- Defense Advanced Research Projects Agency
- Keck Foundation
- McKnight Foundation
- Gatsby Charitable Foundation
- Stanford Medical Scientist Training Program
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Diverse optogenetic tools have allowed versatile control over neural activity. Many depolarizing and hyperpolarizing tools have now been developed in multiple laboratories and tested across different preparations, presenting opportunities but also making it difficult to draw direct comparisons. This challenge has been compounded by the dependence of performance on parameters such as vector, promoter, expression time, illumination, cell type and many other variables. As a result, it has become increasingly complicated for end users to select the optimal reagents for their experimental needs. For a rapidly growing field, critical figurer. of merit should be formalized both to establish a framework for further development and so that end users can readily understand how these standardized parameters translate into performance. Here we systematically compared microbial opsins under matched experimental conditions to extract essential principles and identify key parameters for the conduct, design and interpretation of experiments involving optogenetic techniques.
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