Journal
NATURE METHODS
Volume 8, Issue 10, Pages 837-U99Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1700
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Funding
- Cancer Research Institute
- American Cancer Society
- Howard Hughes Medical Institute
- US National Institutes of Health [GM55040, GM62583, EY016546, P50GM081879]
- National Institutes of Health [GM084040]
- Searle Scholars Fellowship
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The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
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