Journal
NATURE METHODS
Volume 8, Issue 7, Pages 568-U70Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1622
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Funding
- Dana Foundation
- Hertie Foundation
- Christopher and Dana Reeve Foundation
- US National Institutes of Health
- Deutsche Forschungsgemeinschaft [SFB 571, SFB 870, SFB 596]
- German Federal Ministry of Research and Education (Competence Network Multiple Sclerosis)
- Institute of Advanced Study (Technische Universitat Munchen)
- Bundesministierium fur Bildung und Forschung (ERA-Net 'two-photon imaging')
- Alexander von Humboldt Foundation
- Center for Integrated Protein Science (Munich)
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The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.
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