4.8 Article

Micropilot: automation of fluorescence microscopy-based imaging for systems biology

Journal

NATURE METHODS
Volume 8, Issue 3, Pages 246-+

Publisher

NATURE RESEARCH
DOI: 10.1038/NMETH.1558

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Funding

  1. European Commission [LSHG-CT-2004-503464, FP7/2007-2013-241548, FP7/2007-2013-258068]
  2. Landesstiftung Baden-Wurttemberg [01GS0865]
  3. Deutsche Forschungsgemeinschaft [Graduiertenkolleg GRK118]

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Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.

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