Journal
NATURE METHODS
Volume 8, Issue 11, Pages 933-935Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1716
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Funding
- US National Institutes of Health [R01 GM080148]
- National Institutes of Health [5T32HG002760]
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We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables large-scale, multiplexed quantitative proteomics using isobaric tagging.
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