Journal
NATURE METHODS
Volume 8, Issue 12, Pages 1037-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1740
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Funding
- Australian Stem Cell Centre
- National Health and Medical Research Council of Australia [606586, 573707]
- National Heart Foundation (Australia) [G 08M 3711, CR 08S 3958]
- Netherlands Organization for Scientific Research
- Netherlands Institute of Regenerative Medicine
- Victorian State Government
- National Health and Medical Research Council of Australia's Independent Research Institutes
- Marie Curie Co-fund Action
- National Health and Medical Research Council of Australia
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NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
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