Journal
NATURE METHODS
Volume 8, Issue 10, Pages 849-U114Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1679
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Funding
- Wellcome Trust [083524/Z/07/Z, 073980/Z/03/Z, 08136/Z/03/Z, 0909444/Z/09/Z]
- EU FP7 Prospects network [HEALTH-F4-2008-201648]
- Royal Society of Edinburgh
- Association for International Cancer Research
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We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.
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