Journal
NATURE METHODS
Volume 8, Issue 2, Pages 143-U63Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1553
Keywords
-
Categories
Funding
- Marie Curie excellence grant [MEXT-CT-2006-042334]
- Spanish Ministry of Science and Innovation [BFU2008-03237, CONSOLIDER CSD2009-00088]
Ask authors/readers for more resources
Time resolution of current single-molecule fluorescence techniques is limited to milliseconds because of dye blinking and bleaching. Here we introduce a photoprotection strategy that affords microsecond resolution by combining efficient triplet quenching by oxygen and Trolox with minimized bleaching via the oxygen radical scavenger cysteamine. Using this approach we resolved the single-molecule microsecond conformational fluctuations of two proteins: the two-state folder alpha-spectrin SH3 domain and the ultrafast downhill folder BBL.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available