Journal
NATURE METHODS
Volume 8, Issue 7, Pages 592-U112Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1625
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Funding
- Howard Hughes Medical Institute's JFRC
- JFRC
- National Science and Engineering Research Council of Canada
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We designed a real-time computer vision system, the Multi-Worm Tracker (MWT), which can simultaneously quantify the behavior of dozens of Caenorhabditis elegans on a Petri plate at video rates. We examined three traditional behavioral paradigms using this system: spontaneous movement on food, where the behavior changes over tens of minutes; chemotaxis, where turning events must be detected accurately to determine strategy; and habituation of response to tap, where the response is stochastic and changes over time. In each case, manual analysis or automated single-worm tracking would be tedious and time-consuming, but the MWT system allowed rapid quantification of behavior with minimal human effort. Thus, this system will enable large-scale forward and reverse genetic screens for complex behaviors.
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