Journal
NATURE METHODS
Volume 7, Issue 5, Pages 387-U70Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1452
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Funding
- EUREKA [R01GM085457]
- US National Institute of Health [P50GM68762]
- US Army Research Office [DAAD1903D0004]
- Natural Sciences and Engineering Research Council of Canada
- Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BD/47736/2008]
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We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
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