Journal
NATURE METHODS
Volume 7, Issue 5, Pages 383-U64Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1446
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Funding
- Humboldt Foundation
- European Commission [health-F4-2008-201648/PROSPECTS]
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We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.
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