Journal
NATURE METHODS
Volume 7, Issue 3, Pages 197-U46Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1426
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Funding
- Mallinckrodt Foundation
- US National Institutes of Health (NIH) [DP2OD004437, HL091453-01A1S109, NIH R90 DK 07010301]
- Burroughs Wellcome Foundation
- American Heart Association [0970394N]
- California Institute of Regenerative Medicine [T1-00001, RL100662-1, NIH R21 DE018727, RC1HL100490, NIH R21 DE019274]
- Oak Foundation
- Hagey Laboratory for Pediatric Regenerative Medicine
- [U01HL099776]
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Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCSCs from adult human adipose stem cells.
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