Journal
NATURE METHODS
Volume 7, Issue 7, Pages 508-U33Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1467
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Funding
- Canadian Institutes of Health Research
- German Academic Exchange Service (DAAD)
- MSFHR
- Canada Research Chair
- Canadian Breast Cancer Foundation
- Canada Foundation for Innovation
- Deutsche Forschungsgemeinschaft [SCHI 871/1-1, 871/2-1]
- Michael Smith Foundation for Health Research (MSFHR)
- Swiss National Science Foundation
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As proteome-wide C-terminal sequence analysis has been largely intractable, we developed a polymer-based enrichment approach to profile protein C-terminal peptides by mass spectrometry and identified hundreds of C-terminal peptides in the Escherichia coli proteome. We isotopically labeled GluC protease-digested and undigested samples and identified GluC substrates and their cleavage sites by quantification of neo-C-terminal peptides. Our method thus enables global annotation of protein C-terminal posttranslational modifications, including proteolytic truncations.
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