Journal
NATURE METHODS
Volume 7, Issue 11, Pages 901-U905Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1515
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Funding
- Synthetic Genomics, Inc.
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We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.
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