Journal
NATURE METHODS
Volume 7, Issue 11, Pages 893-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1521
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Funding
- Swiss National Science Foundation
- Marie Curie Intra-European Fellowship
- European Reintegration Grant
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We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
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