Journal
NATURE METHODS
Volume 7, Issue 7, Pages 519-U47Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1465
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Funding
- US National Institutes of Health [R01NS049173, P01AI077774]
- China Agricultural University
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Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrPSc) in brain, spleen, blood and urine of scrapie-affected hamsters.
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