Journal
NATURE METHODS
Volume 7, Issue 8, Pages 623-U18Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1473
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Funding
- US National Institutes of Health [R01 GM081875, GM55694, GM088668]
- Robert A. Welch Foundation [I-1424, I-1544]
- Chicago Community Trust
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Genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. Here we report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of Avena sativa phototropin 1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches.
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