Journal
NATURE METHODS
Volume 7, Issue 3, Pages 209-U66Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1429
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Funding
- Oxford University's Isis Innovation
- UK Biotechnology and Biological Sciences Research Council
- British Heart Foundation
- Biotechnology and Biological Sciences Research Council [BB/F004834/1] Funding Source: researchfish
- BBSRC [BB/F004834/1] Funding Source: UKRI
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We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.
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