Journal
NATURE METHODS
Volume 7, Issue 3, Pages 203-U56Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1421
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Funding
- Burroughs Wellcome Fund
- US National Institutes of Health [GM076039]
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We combined single-molecule fluorescence resonance energy transfer (smFRET) with single-particle tracking in live cells to detect the in vivo conformation of individual proteins. We site-specifically labeled recombinant SNARE proteins with a FRET donor and acceptor before microinjecting them into cultured cells. Individual proteins rapidly incorporated into folded complexes at the cell membrane, demonstrating the potential of this method to reveal dynamic interactions within cells.
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