Journal
NATURE METHODS
Volume 7, Issue 4, Pages 295-298Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1441
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Funding
- European Molecular Biology Organization
- Federation of European Biochemical Societies
- Center for Systems Biology
- European Regional Development Fund
- North-Rhine Westphalia
- EU
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We extend the in vitro principle of co-immunoprecipitation to quantify dynamic protein interactions in living cells. using a multiresolution implementation of fluorescence correlation spectroscopy to achieve maximal temporal resolution, we monitored the interactions of endogenous bait proteins, recruited by quantum dots, with fluorescently tagged prey. With this approach, we analyzed the rapid physiological regulation of protein kinase A.
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