Journal
NATURE METHODS
Volume 7, Issue 11, Pages 923-U84Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1513
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Funding
- Clore Israel Foundation
- Tel Aviv University
- Prajs-Drimmer Institute for the Development of Anti-degenerative Disease Drugs
- Israel Cancer Association
- Canadian Institutes of Health Research
- Canadian Foundation for Innovation
- British Columbia Knowledge Development Fund
- British Columbia Proteomics Network
- Genome Sciences and Technologies graduate program
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Non-cell-autonomous proteins are incorporated into cells that form tight contacts or are invaded by bacteria, but identifying the full repertoire of transferred proteins has been a challenge. Here we introduce a quantitative proteomics approach to sort out non-cell-autonomous proteins synthesized by other cells or intracellular pathogens. Our approach combines stable-isotope labeling of amino acids in cell culture (SILAC), high-purity cell sorting and bioinformatics analysis to identify the repertoire of relevant non-cell-autonomous proteins. This `trans-SILAC' method allowed us to discover many proteins transferred from human B to natural killer cells and to measure biosynthesis rates of Salmonella enterica proteins in infected human cells. Trans-SILAC should be a useful method to examine protein exchange between different cells of multicellular organisms or pathogen and host.
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