Journal
NATURE METHODS
Volume 7, Issue 2, Pages 119-U47Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1416
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Funding
- US National Institutes of Health [T32GM007266]
- Achievement Rewards for College Scientists
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We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.
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