Journal
NATURE METHODS
Volume 6, Issue 5, Pages 359-U60Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1322
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Funding
- Max Planck Society for the Advancement of Science
- High-throughput Epigenetic Regulatory Organization In Chromatin ( HEROIC),
- Role of Ubiquitin and Ubiquitin-like Modifiers in Cellular Regulation (RUBICON)
- European Commission
- Munich Center for Integrated Protein Science ( CIPSM).
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We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
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