Journal
NATURE METHODS
Volume 6, Issue 5, Pages 343-U41Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1318
Keywords
-
Categories
Funding
- Office of Science United States Department of Energy [DE-FG02-02ER63453]
- Synthetic Genomics, Inc
Ask authors/readers for more resources
We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5' exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available