Journal
NATURE METHODS
Volume 6, Issue 4, Pages 275-277Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1314
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Funding
- Ministere de la Recherche and la Fondation pour la Recherche Medicale
- Ligue National Contre le Cancer
- Human Frontier Science Program
- European Network of Excellence DC-THERA
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We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.
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