Journal
NATURE METHODS
Volume 6, Issue 7, Pages 511-U61Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1339
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Funding
- US National Institutes of Health
- US National Institute of Neurological Disorders and Stroke
- GRINTech GmbH
- Stanford Biophysics
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Micro-optics are increasingly used for minimally invasive in vivo imaging, in miniaturized microscopes and in lab-on-a-chip devices. Owing to optical aberrations and lower numerical apertures, a main class of microlens, gradient refractive index lenses, has not achieved resolution comparable to conventional microscopy. Here we describe high-resolution microlenses, and illustrate two-photon imaging of dendritic spines on hippocampal neurons and dual-color nonlinear optical imaging of neuromuscular junctions in live mice.
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