Journal
NATURE METHODS
Volume 6, Issue 8, Pages 603-U70Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1356
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Funding
- National Institutes of Health
- Health-UCLA Vascular Biology training
- [R01EY008152]
- [T32HL69766]
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We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
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