Journal
NATURE METHODS
Volume 6, Issue 3, Pages 203-205Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1302
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Funding
- Eidgenossische Technische Hochschule Zurich
- US National Heart, Lung, and Blood Institute
- National Institutes of Health [N01-HV-28179]
- European Union-funded large integrated FP7 project Systems Biology of T-cell Activation
- F. Hoffmann-La Roche Ltd
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We present a mass spectrometry-based strategy for the absolute quantification of protein complex components isolated through affinity purification. We quantified bait proteins via isotope-labeled reference peptides corresponding to an affinity tag sequence and prey proteins by label-free correlational quantification using the precursor ion signal intensities of proteotypic peptides generated in reciprocal purifications. We used this method to quantitatively analyze interaction stoichiometries in the human protein phosphatase 2A network.
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