Journal
NATURE METHODS
Volume 6, Issue 3, Pages 207-209Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1305
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Funding
- US National Institutes of Health [GM60938, AI50143, GM059380]
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We describe a simple method for efficiently labeling cell-surface sialic acid-containing glycans on living animal cells. The method uses mild periodate oxidation to generate an aldehyde on sialic acids, followed by aniline-catalyzed oxime ligation with a suitable tag. Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of aminooxy-biotin at neutral pH to label the majority of cell-surface sialylated glycoproteins while maintaining high cell viability.
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