Journal
NATURE METHODS
Volume 6, Issue 11, Pages 831-U71Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1380
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Funding
- US National Science Foundation [082265]
- Burroughs-Wellcome Fund Career Awards at the Scientific Interface
- National Institutes of Health Institutional National Research Service Award in Molecular Biophysics [PHS 5 T32 GM08276]
- National Institutes of Health [GM054365]
- Division Of Physics
- Direct For Mathematical & Physical Scien [0822613] Funding Source: National Science Foundation
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We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of similar to 100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.
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