Journal
NATURE METHODS
Volume 7, Issue 1, Pages 47-U7Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1404
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Funding
- EMBO
- Croucher Foundation
- Burroughs Wellcome Fund
- Howard Hughes Medical Institute
- US National Human Genome Research Institute
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R44HG005279, R01HG004401, U54HG004570] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP1OD003958] Funding Source: NIH RePORTER
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Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.
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