Journal
NATURE METHODS
Volume 6, Issue 8, Pages 593-U60Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1354
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Funding
- National Human Genetics Research Institute
- [R01 HG004144]
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We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
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