Journal
NATURE METHODS
Volume 6, Issue 11, Pages 809-U26Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1392
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Funding
- Swedish Research Council [8266]
- Torsten and Ragnar Soderbergs Foundation
- Inga-Britt and Arne Lundberg Research Foundation
- Knut and Alice Wallenberg Foundation
- cNeupro
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We present a method to enrich for glycoproteins from proteomic samples. Sialylated glycoproteins were selectively periodate-oxidized, captured on hydrazide beads, trypsinized and released by acid hydrolysis of sialic acid glycosidic bonds. Mass spectrometric fragment analysis allowed identification of glycan structures, and additional fragmentation of deglycosylated ions yielded peptide sequence information, which allowed glycan attachment site and protein identification. We identified 36 N-linked and 44 O-linked glycosylation sites on glycoproteins from human cerebrospinal fluid.
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