Journal
NATURE METHODS
Volume 6, Issue 6, Pages 439-U57Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1329
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Funding
- National Institutes of Health [HD27056]
- Howard Hughes Medical Institute
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We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.
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