Journal
NATURE METHODS
Volume 6, Issue 8, Pages 600-1349Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1349
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Funding
- US National Institutes of Health [1 R01 GM61936, R01 GM058282, R01 GM084947]
- National Research Service award [1 F32GM67460]
- Swiss National Science Foundation [PBBE33-121069]
- Swiss National Science Foundation (SNF) [PBBE33-121069] Funding Source: Swiss National Science Foundation (SNF)
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In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.
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