Journal
NATURE METHODS
Volume 5, Issue 11, Pages 947-949Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1258
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Funding
- US National Institutes of Health [1P20HG003638, 1R01GM085437, R01GM051426, F32GM080008]
- National Science Foundation Graduate Research Fellowship
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The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
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