Journal
NATURE METHODS
Volume 5, Issue 8, Pages 703-709Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1228
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Funding
- NIGMS NIH HHS [R01 GM072675-04, R01 GM072675, K99 GM083145-01, K99 GM083145] Funding Source: Medline
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We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development.
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