Journal
NATURE METHODS
Volume 5, Issue 11, Pages 955-957Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1264
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Funding
- US National Institutes of Health [R01 EB008087, T32 GM007183, R01 HL56416, R01 HL67384, P01 HL53586]
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A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
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