Journal
NATURE METHODS
Volume 5, Issue 11, Pages 943-945Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1257
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We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
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