4.8 Article

Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex

Journal

NATURE METHODS
Volume 5, Issue 3, Pages 235-237

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1184

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Funding

  1. NHLBI NIH HHS [U01 HL081335-01, U01 HL081335] Funding Source: Medline
  2. NIDDK NIH HHS [P01DK078669, P01 DK078669] Funding Source: Medline
  3. NIGMS NIH HHS [T32GM065103, T32 GM065103] Funding Source: Medline

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We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

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