Journal
NATURE METHODS
Volume 5, Issue 12, Pages 1023-1025Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1267
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Funding
- Wellcome Trust
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Sylamer is a method for detecting microRNA target and small interfering RNA off-target signals in 3 ' untranslated regions from a ranked gene list, sorted from upregulated to downregulated, after a microRNA perturbation or RNA interference experiment. The output is a landscape plot that tracks occurrence biases using hypergeometric P-values for all words across the gene ranking. We demonstrated the utility, speed and accuracy of this approach on several datasets.
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