Journal
NATURE METHODS
Volume 5, Issue 5, Pages 397-399Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1206
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Funding
- NCI NIH HHS [CA101830, R01 CA101830] Funding Source: Medline
- NIGMS NIH HHS [P20 GM072029, R01 GM072670, T32 GM008334, R01 GM072670-01, P20GM072029-01] Funding Source: Medline
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We describe a method to generate monovalent quantum dots (QDs) using agarose gel electrophoresis. We passivated QDs with a carboxy-terminated polyethylene-glycol ligand, yielding particles with half the diameter of commercial QDs, which we conjugated to a single copy of a high-affinity targeting moiety (monovalent streptavidin or antibody to carcinoembryonic antigen) to label cell-surface proteins. The small size improved access of QD-labeled glutamate receptors to neuronal synapses, and monovalency prevented EphA3 tyrosine kinase activation.
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