Journal
NATURE METHODS
Volume 5, Issue 10, Pages 877-879Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1253
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Funding
- US National Institute of Mental Health [MH079197]
- National Institute of General Medicine grants [GM068957, GM077183, GM070357]
- National Science Foundation [PHY0548484, DMS-0603392]
- Burroughs Wellcome Fund Career Award at the Scientific Interface
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM070357, F32GM080966, R01GM068957, R01GM077183] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [R01MH079197] Funding Source: NIH RePORTER
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We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.
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