Journal
NATURE METHODS
Volume 5, Issue 12, Pages 1027-1030Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1271
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Funding
- US National Institute of Allergy and Infectious Diseases [K25-65459]
- National Science Foundation [CHE-0722759]
- University of Maine [GM070358, GM073913]
- National Institute of General Medical Sciences
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Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.
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