Journal
NATURE METHODS
Volume 5, Issue 4, Pages 311-313Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1196
Keywords
-
Categories
Funding
- NIGMS NIH HHS [GM42516, R01 GM042516] Funding Source: Medline
Ask authors/readers for more resources
The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP-proliferating cell nuclear antigen (PCNA).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available